Organ specificity and lactate-dehydrogenase activity. Some properties of human spermatozoal lactate dehydrogenase
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چکیده
منابع مشابه
Some Properties of Human Spermatozoal Lactate Dehydrogenase
1. The presence of a characteristic lactate-dehydrogenase isoenzyme (LD-) in human, mouse and dog testis and in human spermatozoa has been confirmed by electrophoresis on cellulose acetate and on polyacrylamide gel. 2. The human spermatozoal isoenzyme exhibits a much higher affinity for 2-oxobutyrate than any ofthe five isoenzymes found in other tissues. Km values of 0-05mM for pyruvate and 0-1...
متن کاملSome properties of lactate dehydrogenase found in human urine.
1. Urinary lactate dehydrogenase was concentrated and subjected to starchblock electrophoresis. The isoenzyme pattern obtained was shown to be similar to that of the kidney cortex and medulla but different from that of the bladder and kidney pelvis. 2. The values for the relative activity and Michaelis constant of urinary lactate dehydrogenase were similar to those for kidney cortex and medulla...
متن کاملImmunochemical specificity of lactate dehydrogenase-X.
Sperm-producing testes of mammals and some birds contain a unique form of lactate dehydrogenase (EC 1.1.1.27) which is a tetramer composed of C subunits, rather than the A and B subunits of lactate dehydrogenase isozymes 1-5. Immunochemical evidence is presented which confirms the hypothesis that the C subunit is encoded in a separate gene from the A and B polypeptides. The evolutionary relatio...
متن کاملOptimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.
Optimum reaction conditions at 30#{176} ± 0.5#{176} for two continuous spectrophotometric assay procedures, lactate to pyruvate (LP) and pyruvate to lactate (PL), were determined with respect to pH at 30#{176} (pH30) substrate concentration, and coenzyme concentration for the human LDH isoenxymes. For the P L procedure, broad pH30 optima were within the range of 7.20-7.40 for all the LDH isoenz...
متن کاملOrgan Specificity and Lactate-dehydrogenase Activity. Differential Inhibition by Urea and Related Compounds.
1. The effect of urea on the lactate-dehydrogenase activities of human-heart and -liver tissue extracts and on crystalline ox-heart and rabbit-muscle enzyme have been determined. Similar studies on electrophoretically separated isoenzyme fractions have shown an inverse relationship between sensitivity to urea inhibition and electrophoretic mobility. 2. With pyruvate as substrate a sharp change ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1965
ISSN: 0006-2936
DOI: 10.1042/bj0970663